8 computer program , then used to calculate di stance matrix with kimura ' s two - parameter method and construct phylogenetic trees by applying neighbor - joining and maximum parsimony analyses using mega2 . 1 computer program . bootstrap analyses were carried out to evaluate statistical reliability based on 500 resamplings of the data set 新鮮或硅膠干燥樣品被用于總dna提取并用于pcr反應(yīng), pcr擴(kuò)增產(chǎn)物回收后直接用于測序反應(yīng),序列數(shù)據(jù)經(jīng)chstalx1 . 8軟件排序后,采用mega2 . 1軟件根據(jù)kimura ’ s雙因子法計(jì)算遺傳距離,并應(yīng)用鄰接法( neighbour - jointing )和最大簡約法( maximumparsimony )構(gòu)建系統(tǒng)樹, bootstrap法( 500次重復(fù))評估分支可信度。